HILIC of intact ribonuclease B.
Glycoproteins can now be done without compromising between LC resolution and MS sensitivity. Our columns give the highest HILIC resolution of intact glycoproteins in the industry, and they are compatible with MS modifiers.
Glycosylation is a critical quality of mAbs to be characterized, and glycosylation is the hallmark of cancer, making these a source of potential new biomarkers.
HILIC columns are available in 2.1 mm x 50 mm format. Other column dimensions available on request.
bioVidria, Inc. is a research-based company focused on newly invented materials for protein analysis to serve the pharmaceutical, biomedical, and biotechnology markets. The core technology of the company is high purity silica with specialized coatings to direct each application.
We are located in the Purdue Research Park, 1281 Win Hentschel Blvd, West Lafayette, IN 47906.
Polymer core-shell technology is the best of both worlds for protein chromatography: high LC resolution and formic acid compatibility .
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Featured Application Notes and Presentations
Monoclonal antibodies of IgG1 are widely used in the pharmaceutical industry, both as drugs and as antibody-drug conjugates. Free thiols can be introduced by genetic engineering, and the proportion of free-thiol variants can be characterized by RPLC-MS. The challenge is that there are always other that there are always other variants besides the intended ones, hence the need for mass spectrometry to be used in peak identification.
LCMS of monoclonal antibodies, ADCs, and other protein drugs requires new ideas in column design rather than adapting columns from the realm of
small-molecule drugs. The design investigated is a core of solid silica and a shell of dense polymer brush for attaining high resolution in liquid chromatography and high sensitivity in mass spectrometry for protein drugs.